* Never smoked or quit more than one year ago
* Body mass index less than 25 kilograms per square meter
* Physical activity of at least 150 minutes (moderate) or 75 minutes (vigorous) each week
* Four to five of the key components of a healthy diet consistent with current AHA guideline recommendations
* Total cholesterol of less than 200 mg/dL
* Blood pressure below 120/80 mm Hg
* Fasting blood glucose less than 100 mg/dL
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